ABSTRACT
<p><b>OBJECTIVE</b>To probe into the objectivity and authenticity of four properties beteen Mahuang decoction and Maxing Shigan decoction from biothermodynamics.</p><p><b>METHOD</b>The power-time curves of growth of Staphylococcus aureus at different concentrations between Mahuang decoction and Maxing Shigan decoction were determined by TAM Air Isothermal Calorimeter. The growth rate constants of promotive and inhibitory actions were calculated. Moreover, the difference of properties beteen Mahuang decoction and Maxing Shigan decoction was analyzed from the point of view of TCM theory.</p><p><b>RESULT</b>Both the Mahuang decoction and Maxing Shigan decoction could inhibit the growth and metabolism of Staphylococcus aureus. The k and Pm were decreased with the mass increase of the decoction. However, inhibitory activity of Mahuang decoction with warm property was weaker than that of Maxing Shigan decoction with cool property. Moreover, Mahuang decoction decreased heat output in growth metabolism more weakly than Maxing Shigan decoction. There was a stable difference between them.</p><p><b>CONCLUSION</b>Studying on biothermodynamics can show the difference of four properties of Traditional Chinese Medicine. So, it provides a new and useful means for the study of the properties of traditional Chinese medicine.</p>
Subject(s)
Calcium Sulfate , Chemistry , Calorimetry , Cinnamomum , Chemistry , Drug Combinations , Drugs, Chinese Herbal , Chemistry , Pharmacology , Ephedra sinica , Chemistry , Glycyrrhiza uralensis , Chemistry , Materia Medica , Chemistry , Pharmacology , Medicine, Chinese Traditional , Plants, Medicinal , Chemistry , Prunus , Chemistry , Staphylococcus aureus , Metabolism , ThermodynamicsABSTRACT
<p><b>OBJECTIVE</b>To set up the HPLC fingerprints of different samples of processed Rhizoma Coptidi (RC) in order to offer evidence for identifying different processed RC and preliminary discussion on processing mechanism.</p><p><b>METHOD</b>Seven different samples of processed RC were determined by HPLC and the results were analyzed by Hierarchical clustering and similarity evaluation system for chromatographic fingerprint of TCM.</p><p><b>RESULT</b>The characteristic of common peaks for fingerprints of different samples of processed RC is distinct, but there are differences among various processed RC. The different samples of processed RC were classified into two and the result of hierarchical clustering is correlated with the property of traditional Chinese drug.</p><p><b>CONCLUSION</b>It is proved that the method which is convenient, rapid and repeat well can be used to identify different samples of processed RC. The change of property of different processed RC is related with processing adjuvant, the possible mechanism is that new components are produced or the contents of known components have changed due to the adjuvant during processing.</p>
Subject(s)
Acetic Acid , Chromatography, High Pressure Liquid , Methods , Cluster Analysis , Coptis , Chemistry , Drugs, Chinese Herbal , Chemistry , Zingiber officinale , Hot Temperature , Plants, Medicinal , Chemistry , Rhizome , Chemistry , Sodium Chloride, Dietary , Technology, Pharmaceutical , Methods , WineABSTRACT
<p><b>OBJECTIVE</b>To develop a quantitative method for determination of the total organic acids and salicyclic acid in the extract of Radix Isatidis.</p><p><b>METHOD</b>The total organic acids were determined by acid-base titration and the salicylic acid was determined by HPLC.</p><p><b>RESULT</b>It was shown that contents of total organic acids and salicylic acid in the extract of Radix Isatidis were 13.0% and 0.22%, respectively.</p><p><b>CONCLUSION</b>The method can control the quality of this extract effectively and accurately.</p>
Subject(s)
Carboxylic Acids , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Isatis , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Quality Control , Salicylic Acid , Technology, PharmaceuticalABSTRACT
<p><b>OBJECTIVE</b>To observe the different extracts from Radix Isatidis on multiplication of mice lymphocytes.</p><p><b>METHOD</b>Lymphocytes were separated and cultured. Immunological activities of different extracts from Radix Isatidis were studied by thermodynamics and the results were tested by the conventional pharmacological experiments.</p><p><b>RESULT</b>The results showed that the water extract and residue had significant immunological effects while organic solvent extracts had immunological activity to some extent.</p><p><b>CONCLUSION</b>The comparison of immunological activity among the extracts from Radix Isatidis were as follows: residue after extracting > general extract > nBuOH extract > EtOAc extract > CHCl3 extract > P E extract.</p>
Subject(s)
Animals , Male , Mice , Calorimetry , Cell Proliferation , Cells, Cultured , Drugs, Chinese Herbal , Pharmacology , Isatis , Chemistry , Lymphocytes , Plant Roots , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study and analyze the antibacterial effects of different extracts from Radix Isatis.</p><p><b>METHODS</b>Staphylococcus aureus was used as the studied object in the experiment. Antibacterial effects of extracts from Radix Isatis were observed by thermocalrimetry on Staphylococcus aureus, together with common pharmacological experiments.</p><p><b>RESULTS</b>The total extract, ethyl acetate (EtOAc) extract, n-butylalcohol (nBuOH) extract, chloroform (CHCl(3)) extract and petroleum (P.E.) extract had antiviral effects to some extent while the residue after extracting had no antibacterial activity. The potency of antiviral activity among them was as follows: nBuOH extract > EtOAc extract > CHCl(3) extract > total extract > P.E. extract.</p><p><b>CONCLUSION</b>The antibacteriall effects of Radix Isatis were not limited to any active portion, showing that Radix Isatis exerts its antibacterial effects by cooperation of different active fractions in varied ways.</p>